Cleanliness is very important!
Waterborn pathogens (fungi, bacteria, etc.) is one of the main causes for seedling death. Using distilled or boiled water at the same temperature as the flask, fill the flask and gently swish the water around to loosen the babies from the agar mixture.
Then, using a spatula
or plastic knitting needle
, untangle the roots and separate the seedlings. Carefully pour the seedlings into in a bowl of water. Wash as much of the agar off the leaves and roots as you can.
Be very gentle, because the babies are very fragile at this stage. After cleansing, soak the little plants in a diluted fungicide
solution such as Physan, RD 20 or Captan Powder. Remove the seedlings and place them on a clean paper towel
or clean newspaper. At this point you can decide if you want to plant all of them or just the strongest ones.
You can use pots, but I have found shallow trays
work much better for the little orchids first home. The seedlings will grow much better if they are kept together rather than trying to pot each seedling in an individual pot.
Make sure the tray or pot is sterile. Fill it with a medium that will stay moist such as shredded Sphagnum
moss, orchid bark
, or small coconut chips
. Gently wrap some wetted Sphagnum
moss around each seedling root system and place in the tray. When your tray is full, fill in around the little plants with whatever medium you have chosen to use.
Do not overplant. Leave room for air and water to easily access each one of the plants root system.