Gary strongly suggests you use a flasking case or chamber. Gary built his own case and it works very well.
You will also need an area set up to grow your new plants. Temperature and lighting is very important during this period. Gary has built a "growing room" just for his propagations.
Once you have all your tools, materials, and facilities available, you will be ready to proceed.
First, prepare your stem propagation growing medium. Mix the medium according to directions. Boil mixture and then pour into cultural tubes (1 1/2" deep) cook cultural tubes containing the mixture in a pressure cooker or autoclave for 15 to 20 minutes. The pressure of the autoclave or pressure cooker is required to reach the internal sterilizing temperature of 15 PSIG.
Next, prepare a mixture of 3 parts water, 1 part bleach, and a couple of drops of dish soap. Add this mixture to the rectangular plastic tray and fill the spray bottle.
Prepare another mixture of 9 parts water, 1 part bleach, 2 drops of dish soap. Add this mixture to the square flasks.
Sanitation is the most important step of this entire procedure. If you can not provide a sterile area to perform the stem propagation, then don't even try to proceed. Fungus spores in the air will develop and attack the new growth as fast as it grows in a non-sterile environment.
Your area must be at least as clean as a hospital operating room! No kidding! Using the spray bottle of the bleach solution, spray all of the areas inside the flasking case with the bleach solution. You can leave it wet or if you have sterile towels you can dry it.
Place the prepared flasks, culture tubes, tube stoppers, synthetic cotton, forceps, tweezers, razor blades, and tube rack into the prepared flasking case.
: Select the orchid you want to propagate. The orchid must have an inflorescence/flower stem that has just began flowering or has half of the flowers open. Cut the stem from the plant using a sterile razor blade.
Place the cut stem in the prepared rectangle tray of bleach solution.
Gently scrub the stem from the bottom up to the top with the soft sterilized toothbrush
. Don't brush too hard, or you will damage the dormant bud. Place the stem back in the tray.
Place the tray containing the stem into the flasking case.
Once in the case, you can start preparing the stems for propagation. Cut the stem 1" above and below each bud at an angle with a sterile razor blade. Gently remove the covering over the bud with a sterile razor blade. Place the prepared stem in the flask with the bleach mixture and let it soak in the flask for 15 to 20 minutes shaking the flask every 5 minutes.
After the stems are in the tubes, it is best to keep them at about room temperature, 70-75 degrees o
F. This is so the air in the tube does not expand and contract thereby possibly sucking contaminants into the tube.
Remove the stem from the flask and cut off about 1/8" of each end of the s
tem. Using the tweezers, place the stem inside a prepared culture tube, inserting it into the medium until the bud just touches the media. Replace the stopper and set the tube in the growing rack. Place only one cutting in each tube to reduce the risk of contamination.
Labels are very important, make sure you label your tubes so you know what plant is in it. It would be a shame if you have gone through all of these steps just to learn you have no idea what you just propagated.
11: Place the rack into the growing room or the area you have prepared for growing the plants.
If everything is done right, the Plant Gods are happy, and you have no fungus, in a few weeks you will see tiny leaves growing in the tubes. You can expect 1 to 4 growths per bud.
When the leaves are 1/2" to 3/4" long and the roots 1-1/2" long, take the little plants out of their tube and plant in individual little pots or a community pot. I use fine grade coconut husk chips, which are very good for Phals. Do not let the little plants dry out!
If you are successful, your new little plants will look like the beautiful Phalaenopsis
orchids that Gary produced in a couple of years.